Review



human pan monocyte isolation kit  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Miltenyi Biotec human pan monocyte isolation kit
    Human Pan Monocyte Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 768 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pan+monocyte+isolation+kit/Pan+Monocyte+Isolation+Kit%2C+human/pm42505091-89-5-10
    Average 96 stars, based on 768 article reviews
    human pan monocyte isolation kit - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Itaconate negatively regulates innate immunity during fungal pneumonia.
    Article Snippet: Peripheral blood samples (10 mL) from healthy human volunteers were drawn into BD Vacutainer Blood Collection Tubes (EDTA K2) and peripheral blood mononuclear cells isolated via Ficoll-Paque PLUS (MilliporeSigma, Catalog #GE17-1440-02). .. Monocytes were isolated using a human Pan Monocyte Isolation Kit (Miltenyi Biotec Catalog #130-096-537) and cultured with human recombinant M-CSF (50 ng/mL; STEMCELL Technologies Catalog #78057) and ImmunoCult-SF Macrophage Medium (STEMCELL Technologies, Catalog #10961) for 4 days. ..

    Article Title: Inflammasome formation and interleukin-1β secretion are reduced in peripheral blood monocytes from HIV+ cannabis users.
    Article Snippet: Chronic systemic inflammation remains a defining feature of human immunodeficiency virus (HIV) infection, partly driven by the translocation of microbial-derived products, including toll-like receptor 4 agonist lipopolysaccharide, from the gut, which is a well established HIV reservoir.. These products activate peripheral blood monocytes, leading to the secretion of proinflammatory cytokines, particularly interleukin-1ß (IL-1ß), which exacerbates systemic inflammation.. Cannabis and its bioactive constituents, Δ 9 -tetrahydrocannabinol (THC) and cannabidiol, exhibit immune-modulating properties, yet their effects on innate immune pathways in HIV remain poorly defined.

    Article Title: Nasal administration of Protollin enhances monocyte phagocytosis and decreases CD8 + T cell cytotoxicity in subjects with early Alzheimer's disease: a Phase 1 clinical trial.
    Article Snippet: .. We proceeded to monocyte enrichment using the Human Pan Monocyte Isolation kit (Miltenyi Biotec, catalogue number 130-096-537) according to the manufacturer's protocol. .. Untouched, enriched monocytes and non-monocytes were collected and stained with Human TruStain FcX (Biolegend, catalogue number 422302, 1:20 dilution) for 15 minutes at 4°C.

    Article Title: Macrophage Phenotype Alterations in Treated Periodontitis Patients Suggest a Pro-Inflammatory Bias.
    Article Snippet: .. Then, PBMCs underwent negative magnetic cell separation (Human Pan Monocyte Isolation Kit, Miltenyi Biotec, NRW, Germany) for monocyte isolation. .. An anti- CD14- APC monoclonal antibody (clone M5E2; BD Biosciences, NJ, USA) confirmed a highly enriched monocyte population via flow cytometry (3.5- fold enrichment).

    Article Title: Δ 9 -Tetrahydrocannabinol (THC) and Cannabidiol (CBD) Diminish CD16 + Monocyte-Induced Astrocyte Inflammation, while THC Uniquely Inhibits Monocyte Chemotaxis Independent of HIV Status.
    Article Snippet: PBMCs were isolated by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare Life Sciences). .. Pan monocytes were isolated from PBMCs by negative magnetic selection utilizing the Human Pan Monocyte Isolation Kit per the manufacturer (Miltenyi Biotec, Begisch Gladbach, Germany). .. For the isolation of CD16+ monocytes, pan monocytes were first stained with anti-CD16 (PE) for 15 min on ice, followed by positive magnetic selection using MojoSortTM Human anti-PE Nanobeads (Biolegend), according to the manufacturer’s instructions.

    Article Title: Interleukin-36 upregulates type-I interferon responses in systemic lupus erythematosus by promoting the accumulation of self-nucleic acids
    Article Snippet: .. Monocytes were purified from PBMCs using a human pan-monocyte isolation kit (Miltenyi Biotech). .. Cells were cultured in RPMI 1640 supplemented with 1% Penicillin/Streptomycin, 10% FBS, 1X MEM Non-Essential Amino Acids (Gibco) and 1M HEPES Buffer Solution (Gibco).

    Article Title: mRNA vaccination overcomes haemozoin-mediated impairment of whole-parasite malaria vaccines in mice
    Article Snippet: .. Monocytes were enriched from peripheral blood mononuclear cells using a Miltenyi human pan monocyte isolation kit and plated in differentiation medium (RPMI supplemented with 10% FBS, 1% pen–strep, 1% sodium pyruvate, 1% HEPES, 1% non-essential amino acids, 50 ng ml −1 GM-CSF and 50 ng ml −1 IL-4) for 6 days. ..

    Article Title: Modulatory effect of magnesium sulfate on NLRP3 inflammasome expression by human monocytes stimulated in vitro with monosodium urate.
    Article Snippet: After these procedures, the cells were resuspended in RPMI 1640 culture medium (Sigma-Aldrich) supplemented with 10% inactivated fetal bovine serum (complete medium) and counted with Trypan blue solution (1:1 v/v). .. After counting, the cell concentration was adjusted to 1x107 PBMCs/mL for the monocyte separation, and the magnetic beads from the Human Pan Monocyte Isolation Kit (Miltenyi Biotec, Germany) were employed according to the manufacturer's instructions. ..

    Cell Culture:

    Article Title: Itaconate negatively regulates innate immunity during fungal pneumonia.
    Article Snippet: Peripheral blood samples (10 mL) from healthy human volunteers were drawn into BD Vacutainer Blood Collection Tubes (EDTA K2) and peripheral blood mononuclear cells isolated via Ficoll-Paque PLUS (MilliporeSigma, Catalog #GE17-1440-02). .. Monocytes were isolated using a human Pan Monocyte Isolation Kit (Miltenyi Biotec Catalog #130-096-537) and cultured with human recombinant M-CSF (50 ng/mL; STEMCELL Technologies Catalog #78057) and ImmunoCult-SF Macrophage Medium (STEMCELL Technologies, Catalog #10961) for 4 days. ..

    Recombinant:

    Article Title: Itaconate negatively regulates innate immunity during fungal pneumonia.
    Article Snippet: Peripheral blood samples (10 mL) from healthy human volunteers were drawn into BD Vacutainer Blood Collection Tubes (EDTA K2) and peripheral blood mononuclear cells isolated via Ficoll-Paque PLUS (MilliporeSigma, Catalog #GE17-1440-02). .. Monocytes were isolated using a human Pan Monocyte Isolation Kit (Miltenyi Biotec Catalog #130-096-537) and cultured with human recombinant M-CSF (50 ng/mL; STEMCELL Technologies Catalog #78057) and ImmunoCult-SF Macrophage Medium (STEMCELL Technologies, Catalog #10961) for 4 days. ..

    Magnetic Cell Separation:

    Article Title: Macrophage Phenotype Alterations in Treated Periodontitis Patients Suggest a Pro-Inflammatory Bias.
    Article Snippet: .. Then, PBMCs underwent negative magnetic cell separation (Human Pan Monocyte Isolation Kit, Miltenyi Biotec, NRW, Germany) for monocyte isolation. .. An anti- CD14- APC monoclonal antibody (clone M5E2; BD Biosciences, NJ, USA) confirmed a highly enriched monocyte population via flow cytometry (3.5- fold enrichment).

    Selection:

    Article Title: Δ 9 -Tetrahydrocannabinol (THC) and Cannabidiol (CBD) Diminish CD16 + Monocyte-Induced Astrocyte Inflammation, while THC Uniquely Inhibits Monocyte Chemotaxis Independent of HIV Status.
    Article Snippet: PBMCs were isolated by density gradient centrifugation using Ficoll-Paque PLUS (GE Healthcare Life Sciences). .. Pan monocytes were isolated from PBMCs by negative magnetic selection utilizing the Human Pan Monocyte Isolation Kit per the manufacturer (Miltenyi Biotec, Begisch Gladbach, Germany). .. For the isolation of CD16+ monocytes, pan monocytes were first stained with anti-CD16 (PE) for 15 min on ice, followed by positive magnetic selection using MojoSortTM Human anti-PE Nanobeads (Biolegend), according to the manufacturer’s instructions.

    Purification:

    Article Title: Interleukin-36 upregulates type-I interferon responses in systemic lupus erythematosus by promoting the accumulation of self-nucleic acids
    Article Snippet: .. Monocytes were purified from PBMCs using a human pan-monocyte isolation kit (Miltenyi Biotech). .. Cells were cultured in RPMI 1640 supplemented with 1% Penicillin/Streptomycin, 10% FBS, 1X MEM Non-Essential Amino Acids (Gibco) and 1M HEPES Buffer Solution (Gibco).

    Concentration Assay:

    Article Title: Modulatory effect of magnesium sulfate on NLRP3 inflammasome expression by human monocytes stimulated in vitro with monosodium urate.
    Article Snippet: After these procedures, the cells were resuspended in RPMI 1640 culture medium (Sigma-Aldrich) supplemented with 10% inactivated fetal bovine serum (complete medium) and counted with Trypan blue solution (1:1 v/v). .. After counting, the cell concentration was adjusted to 1x107 PBMCs/mL for the monocyte separation, and the magnetic beads from the Human Pan Monocyte Isolation Kit (Miltenyi Biotec, Germany) were employed according to the manufacturer's instructions. ..

    Magnetic Beads:

    Article Title: Modulatory effect of magnesium sulfate on NLRP3 inflammasome expression by human monocytes stimulated in vitro with monosodium urate.
    Article Snippet: After these procedures, the cells were resuspended in RPMI 1640 culture medium (Sigma-Aldrich) supplemented with 10% inactivated fetal bovine serum (complete medium) and counted with Trypan blue solution (1:1 v/v). .. After counting, the cell concentration was adjusted to 1x107 PBMCs/mL for the monocyte separation, and the magnetic beads from the Human Pan Monocyte Isolation Kit (Miltenyi Biotec, Germany) were employed according to the manufacturer's instructions. ..



    Similar Products

    96
    Miltenyi Biotec human pan monocyte isolation kit
    Human Pan Monocyte Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pan+monocyte+isolation+kit/Pan+Monocyte+Isolation+Kit%2C+human/pm42505091-89-5-10
    Average 96 stars, based on 1 article reviews
    human pan monocyte isolation kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec pan monocyte isolation kit
    Pan Monocyte Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pan+monocyte+isolation+kit/Pan+Monocyte+Isolation+Kit%2C+human/pm42471470-52-11-15
    Average 96 stars, based on 1 article reviews
    pan monocyte isolation kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec antibody cocktail
    Antibody Cocktail, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pan+monocyte+isolation+kit/Pan+Monocyte+Isolation+Kit%2C+human/pm42401537-324-24-32
    Average 96 stars, based on 1 article reviews
    antibody cocktail - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec magnetic separation kit
    Magnetic Separation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pan+monocyte+isolation+kit/Pan+Monocyte+Isolation+Kit%2C+human/pmc12924898-249-4-7
    Average 96 stars, based on 1 article reviews
    magnetic separation kit - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results